Evaluation of Reference Genes to Analyze Gene Expression in Silverside Odontesthes humensis Under Different Environmental Conditions

Evaluation of Reference Genes to Analyze Gene Expression in Silverside Odontesthes humensis Under Different Environmental Conditions

Some mammalian reference genes, which are widely used to normalize the qRT-PCR, could not be used for this purpose due to its high expression variation. The normalization with false reference genes leads to misinterpretation of results. The silversides (Odontesthes spp.) has been used as models for...

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Bibliographic Details
Main Authors: Tony L. R. Silveira, William B. Domingues, Mariana H. Remião, Lucas Santos, Bruna Barreto, Ingrid M. Lessa, Antonio Sergio Varela Junior, Diego Martins Pires, Carine Corcini, Tiago Collares, Fabiana K. Seixas, Ricardo B. Robaldo, Vinicius F. Campos
Format: Article
Language:English
Published: Frontiers Media S.A. 2018-03-01
Series:Frontiers in Genetics
Subjects:
algorithm
expression
fish
gene
normalization
real time PCR
Online Access:http://journal.frontiersin.org/article/10.3389/fgene.2018.00075/full

Internet

http://journal.frontiersin.org/article/10.3389/fgene.2018.00075/full

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