A novel Ancestral Beijing sublineage of Mycobacterium tuberculosis suggests the transition site to Modern Beijing sublineages
Abstract Global Mycobacterium tuberculosis population comprises 7 major lineages. The Beijing strains, particularly the ones classified as Modern groups, have been found worldwide, frequently associated with drug resistance, younger ages, outbreaks and appear to be expanding. Here, we report analysi...
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2019-09-01
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doaj-964f85b92aeb487a8af930403f2868dd2020-12-08T08:09:12ZengNature Publishing GroupScientific Reports2045-23222019-09-019111210.1038/s41598-019-50078-3A novel Ancestral Beijing sublineage of Mycobacterium tuberculosis suggests the transition site to Modern Beijing sublineagesPravech Ajawatanawong0Hideki Yanai1Nat Smittipat2Areeya Disratthakit3Norio Yamada4Reiko Miyahara5Supalert Nedsuwan6Worarat Imasanguan7Pacharee Kantipong8Boonchai Chaiyasirinroje9Jiraporn Wongyai10Supada Plitphonganphim11Pornpen Tantivitayakul12Jody Phelan13Julian Parkhill14Taane G. Clark15Martin L. Hibberd16Wuthiwat Ruangchai17Panawun Palittapongarnpim18Tada Juthayothin19Yuttapong Thawornwattana20Wasna Viratyosin21Sissades Tongsima22Surakameth Mahasirimongkol23Katsushi Tokunaga24Prasit Palittapongarnpim25Department of Microbiology, Faculty of Science, Mahidol UniversityFukujuji Hospital, Japan Anti-Tuberculosis Association (JATA)National Center for Genetic Engineering and Biotechnology, National Science and Technology Development AgencyDepartment of Medical Sciences, Ministry of Public HealthResearch Institute of Tuberculosis, JATAGenome Medical Science Project, National Center for Global Health and MedicineChiangrai Prachanukroh Hospital, Ministry of Public HealthChiangrai Prachanukroh Hospital, Ministry of Public HealthChiangrai Prachanukroh Hospital, Ministry of Public HealthTB-HIV Research FoundationTB-HIV Research FoundationDepartment of Biostatistics, Faculty of Public Health, Mahidol UniversityDepartment of Oral Microbiology, Faculty of Dentistry, Mahidol UniversityLondon School of Hygiene and Tropical MedicineWelcome Trust Sanger Institute, HinxtonLondon School of Hygiene and Tropical MedicineLondon School of Hygiene and Tropical MedicineDepartment of Microbiology, Faculty of Science, Mahidol UniversityDepartment of Microbiology, Faculty of Science, Mahidol UniversityNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development AgencyDepartment of Microbiology, Faculty of Science, Mahidol UniversityNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development AgencyNational Center for Genetic Engineering and Biotechnology, National Science and Technology Development AgencyDepartment of Medical Sciences, Ministry of Public HealthGenome Medical Science Project, National Center for Global Health and MedicineDepartment of Microbiology, Faculty of Science, Mahidol UniversityAbstract Global Mycobacterium tuberculosis population comprises 7 major lineages. The Beijing strains, particularly the ones classified as Modern groups, have been found worldwide, frequently associated with drug resistance, younger ages, outbreaks and appear to be expanding. Here, we report analysis of whole genome sequences of 1170 M. tuberculosis isolates together with their patient profiles. Our samples belonged to Lineage 1–4 (L1–L4) with those of L1 and L2 being equally dominant. Phylogenetic analysis revealed several new or rare sublineages. Differential associations between sublineages of M. tuberculosis and patient profiles, including ages, ethnicity, HIV (human immunodeficiency virus) infection and drug resistance were demonstrated. The Ancestral Beijing strains and some sublineages of L4 were associated with ethnic minorities while L1 was more common in Thais. L2.2.1.Ancestral 4 surprisingly had a mutation that is typical of the Modern Beijing sublineages and was common in Akha and Lahu tribes who have migrated from Southern China in the last century. This may indicate that the evolutionary transition from the Ancestral to Modern Beijing sublineages might be gradual and occur in Southern China, where the presence of multiple ethnic groups might have allowed for the circulations of various co-evolving sublineages which ultimately lead to the emergence of the Modern Beijing strains.https://doi.org/10.1038/s41598-019-50078-3 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Pravech Ajawatanawong Hideki Yanai Nat Smittipat Areeya Disratthakit Norio Yamada Reiko Miyahara Supalert Nedsuwan Worarat Imasanguan Pacharee Kantipong Boonchai Chaiyasirinroje Jiraporn Wongyai Supada Plitphonganphim Pornpen Tantivitayakul Jody Phelan Julian Parkhill Taane G. Clark Martin L. Hibberd Wuthiwat Ruangchai Panawun Palittapongarnpim Tada Juthayothin Yuttapong Thawornwattana Wasna Viratyosin Sissades Tongsima Surakameth Mahasirimongkol Katsushi Tokunaga Prasit Palittapongarnpim |
spellingShingle |
Pravech Ajawatanawong Hideki Yanai Nat Smittipat Areeya Disratthakit Norio Yamada Reiko Miyahara Supalert Nedsuwan Worarat Imasanguan Pacharee Kantipong Boonchai Chaiyasirinroje Jiraporn Wongyai Supada Plitphonganphim Pornpen Tantivitayakul Jody Phelan Julian Parkhill Taane G. Clark Martin L. Hibberd Wuthiwat Ruangchai Panawun Palittapongarnpim Tada Juthayothin Yuttapong Thawornwattana Wasna Viratyosin Sissades Tongsima Surakameth Mahasirimongkol Katsushi Tokunaga Prasit Palittapongarnpim A novel Ancestral Beijing sublineage of Mycobacterium tuberculosis suggests the transition site to Modern Beijing sublineages Scientific Reports |
author_facet |
Pravech Ajawatanawong Hideki Yanai Nat Smittipat Areeya Disratthakit Norio Yamada Reiko Miyahara Supalert Nedsuwan Worarat Imasanguan Pacharee Kantipong Boonchai Chaiyasirinroje Jiraporn Wongyai Supada Plitphonganphim Pornpen Tantivitayakul Jody Phelan Julian Parkhill Taane G. Clark Martin L. Hibberd Wuthiwat Ruangchai Panawun Palittapongarnpim Tada Juthayothin Yuttapong Thawornwattana Wasna Viratyosin Sissades Tongsima Surakameth Mahasirimongkol Katsushi Tokunaga Prasit Palittapongarnpim |
author_sort |
Pravech Ajawatanawong |
title |
A novel Ancestral Beijing sublineage of Mycobacterium tuberculosis suggests the transition site to Modern Beijing sublineages |
title_short |
A novel Ancestral Beijing sublineage of Mycobacterium tuberculosis suggests the transition site to Modern Beijing sublineages |
title_full |
A novel Ancestral Beijing sublineage of Mycobacterium tuberculosis suggests the transition site to Modern Beijing sublineages |
title_fullStr |
A novel Ancestral Beijing sublineage of Mycobacterium tuberculosis suggests the transition site to Modern Beijing sublineages |
title_full_unstemmed |
A novel Ancestral Beijing sublineage of Mycobacterium tuberculosis suggests the transition site to Modern Beijing sublineages |
title_sort |
novel ancestral beijing sublineage of mycobacterium tuberculosis suggests the transition site to modern beijing sublineages |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2019-09-01 |
description |
Abstract Global Mycobacterium tuberculosis population comprises 7 major lineages. The Beijing strains, particularly the ones classified as Modern groups, have been found worldwide, frequently associated with drug resistance, younger ages, outbreaks and appear to be expanding. Here, we report analysis of whole genome sequences of 1170 M. tuberculosis isolates together with their patient profiles. Our samples belonged to Lineage 1–4 (L1–L4) with those of L1 and L2 being equally dominant. Phylogenetic analysis revealed several new or rare sublineages. Differential associations between sublineages of M. tuberculosis and patient profiles, including ages, ethnicity, HIV (human immunodeficiency virus) infection and drug resistance were demonstrated. The Ancestral Beijing strains and some sublineages of L4 were associated with ethnic minorities while L1 was more common in Thais. L2.2.1.Ancestral 4 surprisingly had a mutation that is typical of the Modern Beijing sublineages and was common in Akha and Lahu tribes who have migrated from Southern China in the last century. This may indicate that the evolutionary transition from the Ancestral to Modern Beijing sublineages might be gradual and occur in Southern China, where the presence of multiple ethnic groups might have allowed for the circulations of various co-evolving sublineages which ultimately lead to the emergence of the Modern Beijing strains. |
url |
https://doi.org/10.1038/s41598-019-50078-3 |
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