GMM-Demux: sample demultiplexing, multiplet detection, experiment planning, and novel cell-type verification in single cell sequencing

GMM-Demux: sample demultiplexing, multiplet detection, experiment planning, and novel cell-type verification in single cell sequencing

Abstract Identifying and removing multiplets are essential to improving the scalability and the reliability of single cell RNA sequencing (scRNA-seq). Multiplets create artificial cell types in the dataset. We propose a Gaussian mixture model-based multiplet identification method, GMM-Demux. GMM-Dem...

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Bibliographic Details
Main Authors: Hongyi Xin, Qiuyu Lian, Yale Jiang, Jiadi Luo, Xinjun Wang, Carla Erb, Zhongli Xu, Xiaoyi Zhang, Elisa Heidrich-O’Hare, Qi Yan, Richard H. Duerr, Kong Chen, Wei Chen
Format: Article
Language:English
Published: BMC 2020-07-01
Series:Genome Biology
Subjects:
Single cell RNA
Multiplets
Rare cell type
Phony cell type
Demultiplex
Sample barcoding
Online Access:http://link.springer.com/article/10.1186/s13059-020-02084-2

Internet

http://link.springer.com/article/10.1186/s13059-020-02084-2

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