Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples.

Early MinION™ nanopore single-molecule sequencing technology enables the characterization of hepatitis B virus genetic complexity in clinical samples.

Until recently, the method of choice to characterize viral diversity consisted in cloning PCR amplicons of full-length viral genomes and Sanger-sequencing of multiple clones. However, this is extremely laborious, time-consuming, and low-throughput. Next generation short-read sequencing appears also...

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Bibliographic Details
Main Authors: Virginie Sauvage, Laure Boizeau, Daniel Candotti, Mathias Vandenbogaert, Annabelle Servant-Delmas, Valérie Caro, Syria Laperche
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2018-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC5864009?pdf=render

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http://europepmc.org/articles/PMC5864009?pdf=render

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