A Chimeric Egfr Protein Reporter Mouse Reveals Egfr Localization and Trafficking In Vivo

A Chimeric Egfr Protein Reporter Mouse Reveals Egfr Localization and Trafficking In Vivo

EGF receptor (EGFR) is a critical signaling node throughout life. However, it has not been possible to directly visualize endogenous Egfr in mice. Using CRISPR/Cas9 genome editing, we appended a fluorescent reporter to the C terminus of the Egfr. Homozygous reporter mice appear normal and EGFR signa...

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Bibliographic Details
Main Authors: Yu-Ping Yang, Haiting Ma, Alina Starchenko, Won Jae Huh, Wei Li, F. Edward Hickman, Qin Zhang, Jeffrey L. Franklin, Douglas P. Mortlock, Sabine Fuhrmann, Bruce D. Carter, Rebecca A. Ihrie, Robert J. Coffey
Format: Article
Language:English
Published: Elsevier 2017-05-01
Series:Cell Reports
Subjects:
Egfr
protein reporter
CRISPR/Cas9
amphiregulin
intracellular trafficking
neural stem cells
intestinal stem cells
Online Access:http://www.sciencedirect.com/science/article/pii/S2211124717305429

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http://www.sciencedirect.com/science/article/pii/S2211124717305429

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