Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions
Abstract The extracellular matrix architecture is composed of supramolecular fibrillar networks that define tissue specific cellular microenvironments. Hemicentins (Hmcn1 and Hmcn2) are ancient and very large members (> 600 kDa) of the fibulin family, whose short members are known to guide proper...
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doaj-96f15bdd865a41c29b23c156bb4759bc2021-09-12T11:22:15ZengNature Publishing GroupScientific Reports2045-23222021-09-0111111610.1038/s41598-021-96824-4Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctionsDaniela Welcker0Cornelia Stein1Natalia Martins Feitosa2Joy Armistead3Jin-Li Zhang4Steffen Lütke5Andre Kleinridders6Jens C. Brüning7Sabine A. Eming8Gerhard Sengle9Anja Niehoff10Wilhelm Bloch11Matthias Hammerschmidt12Institute of Zoology, Developmental Biology Unit, University of CologneInstitute of Zoology, Developmental Biology Unit, University of CologneInstitute of Zoology, Developmental Biology Unit, University of CologneInstitute of Zoology, Developmental Biology Unit, University of CologneInstitute of Zoology, Developmental Biology Unit, University of CologneCenter for Biochemistry, University of CologneDepartment Neuronal Control of Metabolism, Max-Planck Institute for Metabolism ResearchDepartment Neuronal Control of Metabolism, Max-Planck Institute for Metabolism ResearchInstitute of Zoology, Developmental Biology Unit, University of CologneCenter for Molecular Medicine Cologne (CMMC), University of CologneCologne Center for Musculoskeletal Biomechanics (CCMB), University of CologneInstitute of Cardiology and Sports Medicine, German Sport University CologneInstitute of Zoology, Developmental Biology Unit, University of CologneAbstract The extracellular matrix architecture is composed of supramolecular fibrillar networks that define tissue specific cellular microenvironments. Hemicentins (Hmcn1 and Hmcn2) are ancient and very large members (> 600 kDa) of the fibulin family, whose short members are known to guide proper morphology and functional behavior of specialized cell types predominantly in elastic tissues. However, the tissue distribution and function of Hemicentins within the cellular microenvironment of connective tissues has remained largely unknown. Performing in situ hybridization and immunofluorescence analyses, we found that mouse Hmcn1 and Hmcn2 show a complementary distribution throughout different tissues and developmental stages. In postnatal dermal–epidermal junctions (DEJ) and myotendinous junctions (MTJ), Hmcn1 is primarily produced by mesenchymal cells (fibroblasts, tenocytes), Hmcn2 by cells of epithelial origin (keratinocytes, myocytes). Hmcn1 −/− mice are viable and show no overt phenotypes in tissue tensile strength and locomotion tests. However, transmission electron microscopy revealed ultrastructural basement membrane (BM) alterations at the DEJ and MTJ of Hmcn1 −/− mice, pointing to a thus far unknown role of Hmcn1 for BM and connective tissue boundary integrity.https://doi.org/10.1038/s41598-021-96824-4 |
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DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daniela Welcker Cornelia Stein Natalia Martins Feitosa Joy Armistead Jin-Li Zhang Steffen Lütke Andre Kleinridders Jens C. Brüning Sabine A. Eming Gerhard Sengle Anja Niehoff Wilhelm Bloch Matthias Hammerschmidt |
spellingShingle |
Daniela Welcker Cornelia Stein Natalia Martins Feitosa Joy Armistead Jin-Li Zhang Steffen Lütke Andre Kleinridders Jens C. Brüning Sabine A. Eming Gerhard Sengle Anja Niehoff Wilhelm Bloch Matthias Hammerschmidt Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions Scientific Reports |
author_facet |
Daniela Welcker Cornelia Stein Natalia Martins Feitosa Joy Armistead Jin-Li Zhang Steffen Lütke Andre Kleinridders Jens C. Brüning Sabine A. Eming Gerhard Sengle Anja Niehoff Wilhelm Bloch Matthias Hammerschmidt |
author_sort |
Daniela Welcker |
title |
Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions |
title_short |
Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions |
title_full |
Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions |
title_fullStr |
Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions |
title_full_unstemmed |
Hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions |
title_sort |
hemicentin-1 is an essential extracellular matrix component of the dermal–epidermal and myotendinous junctions |
publisher |
Nature Publishing Group |
series |
Scientific Reports |
issn |
2045-2322 |
publishDate |
2021-09-01 |
description |
Abstract The extracellular matrix architecture is composed of supramolecular fibrillar networks that define tissue specific cellular microenvironments. Hemicentins (Hmcn1 and Hmcn2) are ancient and very large members (> 600 kDa) of the fibulin family, whose short members are known to guide proper morphology and functional behavior of specialized cell types predominantly in elastic tissues. However, the tissue distribution and function of Hemicentins within the cellular microenvironment of connective tissues has remained largely unknown. Performing in situ hybridization and immunofluorescence analyses, we found that mouse Hmcn1 and Hmcn2 show a complementary distribution throughout different tissues and developmental stages. In postnatal dermal–epidermal junctions (DEJ) and myotendinous junctions (MTJ), Hmcn1 is primarily produced by mesenchymal cells (fibroblasts, tenocytes), Hmcn2 by cells of epithelial origin (keratinocytes, myocytes). Hmcn1 −/− mice are viable and show no overt phenotypes in tissue tensile strength and locomotion tests. However, transmission electron microscopy revealed ultrastructural basement membrane (BM) alterations at the DEJ and MTJ of Hmcn1 −/− mice, pointing to a thus far unknown role of Hmcn1 for BM and connective tissue boundary integrity. |
url |
https://doi.org/10.1038/s41598-021-96824-4 |
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