Transcriptional Activity and Stability of CD39+CD103+CD8+ T Cells in Human High-Grade Endometrial Cancer

• Main Authors: Hagma H. Workel, Nienke van Rooij, Annechien Plat, Diana C.J. Spierings, Rudolf S. N. Fehrmann, Hans W. Nijman, Marco de Bruyn
• Format: Article
• Language: English
• Published: MDPI AG 2020-05-01
• Series: International Journal of Molecular Sciences
• Subjects: tissue-resident memory cell; endometrial cancer; CD103; CD39; transcript stability; cytokinesis
• Online Access: https://www.mdpi.com/1422-0067/21/11/3770
• ISSN: 1661-6596; 1422-0067

Tumor-infiltrating CD8+ T cells (TIL) are of the utmost importance in anti-tumor immunity. CD103 defines tumor-resident memory T cells (T_RM cells) associated with improved survival and response to immune checkpoint blockade (ICB) across human tumors. Co-expression of CD39 and CD103 marks tumor-specific T_RM with enhanced cytolytic potential, suggesting that CD39+CD103+ T_RM could be a suitable biomarker for immunotherapy. However, little is known about the transcriptional activity of T_RM cells in situ. We analyzed CD39+CD103+ T_RM cells sorted from human high-grade endometrial cancers (<i>n </i>= 3) using mRNA sequencing. Cells remained untreated or were incubated with PMA/ionomycin (activation), actinomycin D (a platinum-like chemotherapeutic that inhibits transcription), or a combination of the two. Resting CD39+CD103+ T_RM cells were transcriptionally active and expressed a characteristic T_RM signature. Activated CD39+CD103+ T_RM cells differentially expressed <i>PLEK</i>, <i>TWNK</i>, and <i>FOS</i>, and cytokine genes <i>IFNG</i>, <i>TNF</i>, <i>IL2</i>, <i>CSF2</i> (GM-CSF), and <i>IL21</i>. Findings were confirmed using qPCR and cytokine production was validated by flow cytometry of cytotoxic TIL. We studied transcript stability and found that PMA-responsive genes and mitochondrial genes were particularly stable. In conclusion, CD39+CD103+ T_RM cells are transcriptionally active T_RM cells with a polyfunctional, reactivation-responsive repertoire. Secondly, we hypothesize that differential regulation of transcript stability potentiates rapid responses upon T_RM reactivation in tumors.