Transfection of Babesia bovis by Double Selection with WR99210 and Blasticidin-S and Its Application for Functional Analysis of Thioredoxin Peroxidase-1.

Transfection of Babesia bovis by Double Selection with WR99210 and Blasticidin-S and Its Application for Functional Analysis of Thioredoxin Peroxidase-1.

Genetic manipulation is an essential technique to analyze gene function; however, limited methods are available for Babesia bovis, a causative pathogen of the globally important cattle disease, bovine babesiosis. To date, two stable transfection systems have been developed for B. bovis, using select...

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Bibliographic Details
Main Authors: Masahito Asada, Kazuhide Yahata, Hassan Hakimi, Naoaki Yokoyama, Ikuo Igarashi, Osamu Kaneko, Carlos E Suarez, Shin-ichiro Kawazu
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2015-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4427477?pdf=render

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http://europepmc.org/articles/PMC4427477?pdf=render

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