Novel ecto-tagged integrins reveal their trafficking in live cells

Novel ecto-tagged integrins reveal their trafficking in live cells

Integrins are cell-surface adhesion receptors that are modulated by endo-exocytic trafficking, but existing tools to study this process can interfere with function. Here the authors develop β1 integrins carrying traceable tags in the extracellular domain; a pH-sensitive pHlourin tag or a HaloTag to...

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Main Authors: Clotilde Huet-Calderwood, Felix Rivera-Molina, Daniel V. Iwamoto, Emil B. Kromann, Derek Toomre, David A. Calderwood
Format: Article
Language:English
Published: Nature Publishing Group 2017-09-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-017-00646-w
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spelling doaj-97783d23504d4b7c8a85d54e3bfbedbf2021-05-11T07:45:38ZengNature Publishing GroupNature Communications2041-17232017-09-018111310.1038/s41467-017-00646-wNovel ecto-tagged integrins reveal their trafficking in live cellsClotilde Huet-Calderwood0Felix Rivera-Molina1Daniel V. Iwamoto2Emil B. Kromann3Derek Toomre4David A. Calderwood5Department of Pharmacology, Yale University School of MedicineDepartment of Cell Biology, Yale University School of MedicineDepartment of Pharmacology, Yale University School of MedicineDepartment of Cell Biology, Yale University School of MedicineDepartment of Cell Biology, Yale University School of MedicineDepartment of Pharmacology, Yale University School of MedicineIntegrins are cell-surface adhesion receptors that are modulated by endo-exocytic trafficking, but existing tools to study this process can interfere with function. Here the authors develop β1 integrins carrying traceable tags in the extracellular domain; a pH-sensitive pHlourin tag or a HaloTag to facilitate dye attachment.https://doi.org/10.1038/s41467-017-00646-w
collection DOAJ
language English
format Article
sources DOAJ
author Clotilde Huet-Calderwood
Felix Rivera-Molina
Daniel V. Iwamoto
Emil B. Kromann
Derek Toomre
David A. Calderwood
spellingShingle Clotilde Huet-Calderwood
Felix Rivera-Molina
Daniel V. Iwamoto
Emil B. Kromann
Derek Toomre
David A. Calderwood
Novel ecto-tagged integrins reveal their trafficking in live cells
Nature Communications
author_facet Clotilde Huet-Calderwood
Felix Rivera-Molina
Daniel V. Iwamoto
Emil B. Kromann
Derek Toomre
David A. Calderwood
author_sort Clotilde Huet-Calderwood
title Novel ecto-tagged integrins reveal their trafficking in live cells
title_short Novel ecto-tagged integrins reveal their trafficking in live cells
title_full Novel ecto-tagged integrins reveal their trafficking in live cells
title_fullStr Novel ecto-tagged integrins reveal their trafficking in live cells
title_full_unstemmed Novel ecto-tagged integrins reveal their trafficking in live cells
title_sort novel ecto-tagged integrins reveal their trafficking in live cells
publisher Nature Publishing Group
series Nature Communications
issn 2041-1723
publishDate 2017-09-01
description Integrins are cell-surface adhesion receptors that are modulated by endo-exocytic trafficking, but existing tools to study this process can interfere with function. Here the authors develop β1 integrins carrying traceable tags in the extracellular domain; a pH-sensitive pHlourin tag or a HaloTag to facilitate dye attachment.
url https://doi.org/10.1038/s41467-017-00646-w
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