Characterization of a Minimal pKW2124 Replicon from Weissella cibaria KLC140 and Its Application for the Construction of the Weissella Expression Vector pKUCm1

Characterization of a Minimal pKW2124 Replicon from Weissella cibaria KLC140 and Its Application for the Construction of the Weissella Expression Vector pKUCm1

A 2.1-kb plasmid was previously isolated from Weissella cibaria KLC140 in kimchi and cloned into pUC19 along with the slpA and gfp genes, resulting in an 8.6-kb pKWCSLGFP construct for use as a novel surface display vector. To reduce the size of the vector, the minimal replicon of pKW2124 was determ...

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Bibliographic Details
Main Authors: Hye-Jin eKu, Myeong Soo ePark, Ju-Hoon eLee
Format: Article
Language:English
Published: Frontiers Media S.A. 2015-02-01
Series:Frontiers in Microbiology
Subjects:
Weissella
β-galactosidase
Expression vector
minimal replicon
theta replication
Online Access:http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00035/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00035/full

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