Enzyme activity and thermostability of a non-specific nuclease from Yersinia enterocolitica subsp. palearctica by site-directed mutagenesis

Background: To identify the critical amino acid residues that contribute to the high enzyme activity and good thermostability of Yersinia enterocolitica subsp. palearctica (Y. NSN), 15 mutants of Y. NSN were obtained by site-directed mutagenesis in this study. And their enzyme activity and thermosta...

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Bibliographic Details
Main Authors: Yu Zhang, Zhen-Hua Li, Wei Zheng, Zhen-Xing Tang, Zhi-Liang Zhang, Lu-E Shi
Format: Article
Language:English
Published: Elsevier 2016-11-01
Series:Electronic Journal of Biotechnology
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0717345816300811