Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional Groups

In the 15-mer catalytic core of 10-23 DNAzyme, each residue contributes to the catalytic conformation differently. Here, the critically conserved T4 and the least conserved T8 were modified on their 5-position with hydroxyl, imidazolyl, and amino groups with a hydrogen-bonding ability. These externa...

Full description

Bibliographic Details
Main Authors:	Pengyu Li, Shanshan Du, Yang Li, Junlin He
Format:	Article
Language:	English
Published:	MDPI AG 2017-06-01
Series:	Molecules
Subjects:	10-23 DNAzyme chemical modifications thymine functional group catalytic core observed catalytic constants
Online Access:	http://www.mdpi.com/1420-3049/22/7/1011

id	doaj-97d55d7a6b494295a72417f44db1904e
record_format	Article
spelling	doaj-97d55d7a6b494295a72417f44db1904e2020-11-24T23:25:48ZengMDPI AGMolecules1420-30492017-06-01227101110.3390/molecules22071011molecules22071011Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional GroupsPengyu Li0Shanshan Du1Yang Li2Junlin He3College of Life Sciences, Guizhou University, Guiyang 550025, ChinaCollege of Life Sciences, Guizhou University, Guiyang 550025, ChinaCollege of Life Sciences, Guizhou University, Guiyang 550025, ChinaLaboratory of Bioorganic chemistry, Beijing Institute of Pharmacology and Toxicology, Beijing 100850, ChinaIn the 15-mer catalytic core of 10-23 DNAzyme, each residue contributes to the catalytic conformation differently. Here, the critically conserved T4 and the least conserved T8 were modified on their 5-position with hydroxyl, imidazolyl, and amino groups with a hydrogen-bonding ability. These external functional groups induced new interactions within the catalytic core, resulting in both negative and positive effects on the catalytic activity of 10-23 DNAzyme, and the different linkages could be used to modulate the effect of the functional groups. The conservation of T4 and T8 could be recognized at the level of the nucleobase, but at the level of the functional group, T4 is not completely conserved. Their 5-methyl groups could be modified for a better performance in terms of the DNAzyme.http://www.mdpi.com/1420-3049/22/7/101110-23 DNAzymechemical modificationsthyminefunctional groupcatalytic coreobserved catalytic constants
collection	DOAJ
language	English
format	Article
sources	DOAJ
author	Pengyu Li Shanshan Du Yang Li Junlin He
spellingShingle	Pengyu Li Shanshan Du Yang Li Junlin He Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional Groups Molecules 10-23 DNAzyme chemical modifications thymine functional group catalytic core observed catalytic constants
author_facet	Pengyu Li Shanshan Du Yang Li Junlin He
author_sort	Pengyu Li
title	Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional Groups
title_short	Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional Groups
title_full	Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional Groups
title_fullStr	Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional Groups
title_full_unstemmed	Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional Groups
title_sort	studies on the two thymine residues in the catalytic core of 10-23 dnazyme: the impact on the catalysis of their 5-substituted functional groups
publisher	MDPI AG
series	Molecules
issn	1420-3049
publishDate	2017-06-01
description	In the 15-mer catalytic core of 10-23 DNAzyme, each residue contributes to the catalytic conformation differently. Here, the critically conserved T4 and the least conserved T8 were modified on their 5-position with hydroxyl, imidazolyl, and amino groups with a hydrogen-bonding ability. These external functional groups induced new interactions within the catalytic core, resulting in both negative and positive effects on the catalytic activity of 10-23 DNAzyme, and the different linkages could be used to modulate the effect of the functional groups. The conservation of T4 and T8 could be recognized at the level of the nucleobase, but at the level of the functional group, T4 is not completely conserved. Their 5-methyl groups could be modified for a better performance in terms of the DNAzyme.
topic	10-23 DNAzyme chemical modifications thymine functional group catalytic core observed catalytic constants
url	http://www.mdpi.com/1420-3049/22/7/1011
work_keys_str_mv	AT pengyuli studiesonthetwothymineresiduesinthecatalyticcoreof1023dnazymetheimpactonthecatalysisoftheir5substitutedfunctionalgroups AT shanshandu studiesonthetwothymineresiduesinthecatalyticcoreof1023dnazymetheimpactonthecatalysisoftheir5substitutedfunctionalgroups AT yangli studiesonthetwothymineresiduesinthecatalyticcoreof1023dnazymetheimpactonthecatalysisoftheir5substitutedfunctionalgroups AT junlinhe studiesonthetwothymineresiduesinthecatalyticcoreof1023dnazymetheimpactonthecatalysisoftheir5substitutedfunctionalgroups
_version_	1725555968718143488

Studies on the Two Thymine Residues in the Catalytic Core of 10-23 DNAzyme: The Impact on the Catalysis of Their 5-Substituted Functional Groups

Similar Items