Performance of plate-based cytokine flow cytometry with automated data analysis

<p>Abstract</p> <p>Background</p> <p>Cytokine flow cytometry (CFC) provides a multiparameter alternative to ELISPOT assays for rapid quantitation of antigen-specific T cells. To increase the throughput of CFC assays, we have optimized methods for stimulating, staining,...

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Bibliographic Details
Main Authors: Bredt Barry M, Ghanekar Smita A, Sinclair Elizabeth, Laat Rian de, Orr Patricia L, Suni Maria A, Dunn Holli S, Dunne John F, Maino Vernon C, Maecker Holden T
Format: Article
Language:English
Published: BMC 2003-09-01
Series:BMC Immunology
Online Access:http://www.biomedcentral.com/1471-2172/4/9