Semi-high-throughput detection of Plasmodium falciparum and Plasmodium vivax oocysts in mosquitoes using bead-beating followed by circumsporozoite ELISA and quantitative PCR

Semi-high-throughput detection of Plasmodium falciparum and Plasmodium vivax oocysts in mosquitoes using bead-beating followed by circumsporozoite ELISA and quantitative PCR

Abstract Background The malaria infection status of mosquitoes is commonly determined by microscopic detection of oocysts on the dissected mosquito midgut. This method is labour-intensive, does not allow processing of large numbers of mosquitoes and can be challenging in terms of objective classific...

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Bibliographic Details
Main Authors: Wouter Graumans, Fitsum G. Tadesse, Chiara Andolina, Geert-Jan van Gemert, Karina Teelen, Kjerstin Lanke, Endalamaw Gadisa, Delenasaw Yewhalaw, Marga van de Vegte-Bolmer, Rianne Siebelink-Stoter, Isaïe Reuling, Robert Sauerwein, Teun Bousema
Format: Article
Language:English
Published: BMC 2017-09-01
Series:Malaria Journal
Subjects:
Transmission
Oocyst
Sporozoite
Anopheles
Infectivity
Gametocyte
Online Access:http://link.springer.com/article/10.1186/s12936-017-2011-9

Internet

http://link.springer.com/article/10.1186/s12936-017-2011-9

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