Semi-high-throughput detection of Plasmodium falciparum and Plasmodium vivax oocysts in mosquitoes using bead-beating followed by circumsporozoite ELISA and quantitative PCR
Abstract Background The malaria infection status of mosquitoes is commonly determined by microscopic detection of oocysts on the dissected mosquito midgut. This method is labour-intensive, does not allow processing of large numbers of mosquitoes and can be challenging in terms of objective classific...
Main Authors: | Wouter Graumans, Fitsum G. Tadesse, Chiara Andolina, Geert-Jan van Gemert, Karina Teelen, Kjerstin Lanke, Endalamaw Gadisa, Delenasaw Yewhalaw, Marga van de Vegte-Bolmer, Rianne Siebelink-Stoter, Isaïe Reuling, Robert Sauerwein, Teun Bousema |
---|---|
Format: | Article |
Language: | English |
Published: |
BMC
2017-09-01
|
Series: | Malaria Journal |
Subjects: | |
Online Access: | http://link.springer.com/article/10.1186/s12936-017-2011-9 |
Similar Items
-
Concentration of Plasmodium falciparum gametocytes in whole blood samples by magnetic cell sorting enhances parasite infection rates in mosquito feeding assays
by: Isaie J. Reuling, et al.
Published: (2017-08-01) -
A randomized feasibility trial comparing four antimalarial drug regimens to induce Plasmodium falciparum gametocytemia in the controlled human malaria infection model
by: Isaie J Reuling, et al.
Published: (2018-02-01) -
Cellular immune response of humans to the circumsporozoite protein of Plasmodium vivax
by: Mauricio M. Rodrigues, et al.
Published: (1991-06-01) -
Variants of the Plasmodium vivax circumsporozoite protein (VK210 and VK247) in Colombian isolates
by: JM González, et al.
Published: (2001-07-01) -
Predicting the likelihood and intensity of mosquito infection from sex specific Plasmodium falciparum gametocyte density
by: John Bradley, et al.
Published: (2018-05-01)