Heterologous Expression of Gene Encoded Thermostable Lipase and Lipolytic Activity

Heterologous Expression of Gene Encoded Thermostable Lipase and Lipolytic Activity

Three lipase genes namely LK2, LK3 and LK5 were successfully sub-cloned into expression vector pET30a. The recombinant vectors were heterologically expressed into Escherichia coli BL21 (DE3) by 1 mM IPTG induced at 37 °C. The protein with the size of 32, 31 and 28 kDa with correspond to LK2, LK3 a...

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Bibliographic Details
Main Authors: Nurhasanah, Santi Nurbaiti, Fida Madayanti, Akhmaloka
Format: Article
Language:English
Published: Journal of Pure and Applied Microbiology 2017-03-01
Series:Journal of Pure and Applied Microbiology
Subjects:
thermostable lipase
heterologous expression
lipolytic activity
Online Access:https://microbiologyjournal.org/heterologous-expression-of-gene-encoded-thermostable-lipase-and-lipolytic-activity/

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https://microbiologyjournal.org/heterologous-expression-of-gene-encoded-thermostable-lipase-and-lipolytic-activity/

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