Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome

Complementing 16S rRNA Gene Amplicon Sequencing with Total Bacterial Load To Infer Absolute Species Concentrations in the Vaginal Microbiome

Microbiome studies primarily use 16S rRNA gene amplicon sequencing to assess the relative abundance of bacterial taxa in a community. However, these measurements do not accurately reflect absolute taxon concentrations. We sought to determine whether the product of species’ relative abundance and tot...

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Bibliographic Details
Main Authors: Florencia A. Tettamanti Boshier, Sujatha Srinivasan, Anthony Lopez, Noah G. Hoffman, Sean Proll, David N. Fredricks, Joshua T. Schiffer
Format: Article
Language:English
Published: American Society for Microbiology 2020-04-01
Series:mSystems
Subjects:
applied microbiology
gene amplicon sequencing
quantitative pcr
vaginal microbiome
Online Access:https://doi.org/10.1128/mSystems.00777-19

Internet

https://doi.org/10.1128/mSystems.00777-19

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