Extracting fluorescent reporter time courses of cell lineages from high-throughput microscopy at low temporal resolution.

The extraction of fluorescence time course data is a major bottleneck in high-throughput live-cell microscopy. Here we present an extendible framework based on the open-source image analysis software ImageJ, which aims in particular at analyzing the expression of fluorescent reporters through cell d...

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Bibliographic Details
Main Authors: Mike J Downey, Danuta M Jeziorska, Sascha Ott, T Katherine Tamai, Georgy Koentges, Keith W Vance, Till Bretschneider
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2011-01-01
Series:PLoS ONE
Online Access:https://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22194797/?tool=EBI