Mobile genetic element SCCmec-encoded psm-mec RNA suppresses translation of agrA and attenuates MRSA virulence.
Community acquired-methicillin resistant Staphylococcus aureus (CA-MRSA) is a socially problematic pathogen that infects healthy individuals, causing severe disease. CA-MRSA is more virulent than hospital associated-MRSA (HA-MRSA). The underlying mechanism for the high virulence of CA-MRSA is not kn...
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doaj-9880bc14cbae405ca479e98ad0ded5982020-11-25T00:09:53ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742013-01-0194e100326910.1371/journal.ppat.1003269Mobile genetic element SCCmec-encoded psm-mec RNA suppresses translation of agrA and attenuates MRSA virulence.Chikara KaitoYuki SaitoMariko IkuoYosuke OmaeHan MaoGentaro NaganoTomoko FujiyukiShunsuke NumataXiao HanKazuaki ObataSetsuo HasegawaHiroki YamaguchiKoiti InokuchiTeruyo ItoKeiichi HiramatsuKazuhisa SekimizuCommunity acquired-methicillin resistant Staphylococcus aureus (CA-MRSA) is a socially problematic pathogen that infects healthy individuals, causing severe disease. CA-MRSA is more virulent than hospital associated-MRSA (HA-MRSA). The underlying mechanism for the high virulence of CA-MRSA is not known. The transcription product of the psm-mec gene, located in the mobile genetic element SCCmec of HA-MRSA, but not CA-MRSA, suppresses the expression of phenol-soluble modulin α (PSMα), a cytolytic toxin of S. aureus. Here we report that psm-mec RNA inhibits translation of the agrA gene encoding a positive transcription factor for the PSMα gene via specific binding to agrA mRNA. Furthermore, 25% of 325 clinical MRSA isolates had a mutation in the psm-mec promoter that attenuated transcription, and 9% of the strains had no psm-mec. In most of these psm-mec-mutated or psm-mec-deleted HA-MRSAs, PSMα expression was increased compared with strains carrying intact psm-mec, and some mutated strains produced high amounts of PSMα comparable with that of CA-MRSA. Deletion of psm-mec from HA-MRSA strains carrying intact psm-mec increased the expression of AgrA protein and PSMα, and virulence in mice. Thus, psm-mec RNA suppresses MRSA virulence via inhibition of agrA translation and the absence of psm-mec function in CA-MRSA causes its high virulence property.http://europepmc.org/articles/PMC3617227?pdf=render |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Chikara Kaito Yuki Saito Mariko Ikuo Yosuke Omae Han Mao Gentaro Nagano Tomoko Fujiyuki Shunsuke Numata Xiao Han Kazuaki Obata Setsuo Hasegawa Hiroki Yamaguchi Koiti Inokuchi Teruyo Ito Keiichi Hiramatsu Kazuhisa Sekimizu |
spellingShingle |
Chikara Kaito Yuki Saito Mariko Ikuo Yosuke Omae Han Mao Gentaro Nagano Tomoko Fujiyuki Shunsuke Numata Xiao Han Kazuaki Obata Setsuo Hasegawa Hiroki Yamaguchi Koiti Inokuchi Teruyo Ito Keiichi Hiramatsu Kazuhisa Sekimizu Mobile genetic element SCCmec-encoded psm-mec RNA suppresses translation of agrA and attenuates MRSA virulence. PLoS Pathogens |
author_facet |
Chikara Kaito Yuki Saito Mariko Ikuo Yosuke Omae Han Mao Gentaro Nagano Tomoko Fujiyuki Shunsuke Numata Xiao Han Kazuaki Obata Setsuo Hasegawa Hiroki Yamaguchi Koiti Inokuchi Teruyo Ito Keiichi Hiramatsu Kazuhisa Sekimizu |
author_sort |
Chikara Kaito |
title |
Mobile genetic element SCCmec-encoded psm-mec RNA suppresses translation of agrA and attenuates MRSA virulence. |
title_short |
Mobile genetic element SCCmec-encoded psm-mec RNA suppresses translation of agrA and attenuates MRSA virulence. |
title_full |
Mobile genetic element SCCmec-encoded psm-mec RNA suppresses translation of agrA and attenuates MRSA virulence. |
title_fullStr |
Mobile genetic element SCCmec-encoded psm-mec RNA suppresses translation of agrA and attenuates MRSA virulence. |
title_full_unstemmed |
Mobile genetic element SCCmec-encoded psm-mec RNA suppresses translation of agrA and attenuates MRSA virulence. |
title_sort |
mobile genetic element sccmec-encoded psm-mec rna suppresses translation of agra and attenuates mrsa virulence. |
publisher |
Public Library of Science (PLoS) |
series |
PLoS Pathogens |
issn |
1553-7366 1553-7374 |
publishDate |
2013-01-01 |
description |
Community acquired-methicillin resistant Staphylococcus aureus (CA-MRSA) is a socially problematic pathogen that infects healthy individuals, causing severe disease. CA-MRSA is more virulent than hospital associated-MRSA (HA-MRSA). The underlying mechanism for the high virulence of CA-MRSA is not known. The transcription product of the psm-mec gene, located in the mobile genetic element SCCmec of HA-MRSA, but not CA-MRSA, suppresses the expression of phenol-soluble modulin α (PSMα), a cytolytic toxin of S. aureus. Here we report that psm-mec RNA inhibits translation of the agrA gene encoding a positive transcription factor for the PSMα gene via specific binding to agrA mRNA. Furthermore, 25% of 325 clinical MRSA isolates had a mutation in the psm-mec promoter that attenuated transcription, and 9% of the strains had no psm-mec. In most of these psm-mec-mutated or psm-mec-deleted HA-MRSAs, PSMα expression was increased compared with strains carrying intact psm-mec, and some mutated strains produced high amounts of PSMα comparable with that of CA-MRSA. Deletion of psm-mec from HA-MRSA strains carrying intact psm-mec increased the expression of AgrA protein and PSMα, and virulence in mice. Thus, psm-mec RNA suppresses MRSA virulence via inhibition of agrA translation and the absence of psm-mec function in CA-MRSA causes its high virulence property. |
url |
http://europepmc.org/articles/PMC3617227?pdf=render |
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