Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9

Generation of an ASS1 heterozygous knockout human embryonic stem cell line, WAe001-A-13, using CRISPR/Cas9

The ASS1 gene encodes argininosuccinate synthetase-1, a cytosolic enzyme with a critical role in the urea cycle. Mutations are found in all ASS1 exons and cause the autosomal recessive disorder citrullinemia. Using CRISPR/Cas9-editing, we established the WAe001-A-13 cell line, which was heterozygous...

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Bibliographic Details
Main Authors: Fang Yuan, Dongsheng Guo, Yanli Liu, Yingying Xu, Ge Gao, Yuhang Wu, Fan Yang, Xinrong Ke, Keyu Lai, Hongcheng Wei, Yin-Xiong Li
Format: Article
Language:English
Published: Elsevier 2018-01-01
Series:Stem Cell Research
Online Access:http://www.sciencedirect.com/science/article/pii/S1873506117302386

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http://www.sciencedirect.com/science/article/pii/S1873506117302386

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