Aging Exacerbates Ischemia-Reperfusion-Induced Mitochondrial Respiration Impairment in Skeletal Muscle
Cycles of ischemia-reperfusion (IR) that occur during peripheral arterial disease (PAD) are associated with significant morbi-mortality, and aging is an irreversible risk factor of PAD. However, the effects of advanced age on IR-induced skeletal muscle mitochondrial dysfunction are not well known. Y...
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doaj-98b87bbb4b794739956c96e2981fd0532020-11-25T01:15:33ZengMDPI AGAntioxidants2076-39212019-06-018616810.3390/antiox8060168antiox8060168Aging Exacerbates Ischemia-Reperfusion-Induced Mitochondrial Respiration Impairment in Skeletal MuscleStéphanie Paradis0Anne-Laure Charles1Isabelle Georg2Fabienne Goupilleau3Alain Meyer4Michel Kindo5Gilles Laverny6Daniel Metzger7Bernard Geny8Fédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Institut de Physiologie, Equipe d’Accueil EA3072 “Mitochondrie, Stress Oxydant et Protection Musculaire”, Université de Strasbourg, 67000 Strasbourg, FranceFédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Institut de Physiologie, Equipe d’Accueil EA3072 “Mitochondrie, Stress Oxydant et Protection Musculaire”, Université de Strasbourg, 67000 Strasbourg, FranceFédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Institut de Physiologie, Equipe d’Accueil EA3072 “Mitochondrie, Stress Oxydant et Protection Musculaire”, Université de Strasbourg, 67000 Strasbourg, FranceFédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Institut de Physiologie, Equipe d’Accueil EA3072 “Mitochondrie, Stress Oxydant et Protection Musculaire”, Université de Strasbourg, 67000 Strasbourg, FranceFédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Institut de Physiologie, Equipe d’Accueil EA3072 “Mitochondrie, Stress Oxydant et Protection Musculaire”, Université de Strasbourg, 67000 Strasbourg, FranceFédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Institut de Physiologie, Equipe d’Accueil EA3072 “Mitochondrie, Stress Oxydant et Protection Musculaire”, Université de Strasbourg, 67000 Strasbourg, FranceDepartment of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U1258, CNRS UMR 7104, Université de Strasbourg, 67404 Illkirch, FranceDepartment of Functional Genomics and Cancer, Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), INSERM U1258, CNRS UMR 7104, Université de Strasbourg, 67404 Illkirch, FranceFédération de Médecine Translationnelle de Strasbourg, Faculté de Médecine, Institut de Physiologie, Equipe d’Accueil EA3072 “Mitochondrie, Stress Oxydant et Protection Musculaire”, Université de Strasbourg, 67000 Strasbourg, FranceCycles of ischemia-reperfusion (IR) that occur during peripheral arterial disease (PAD) are associated with significant morbi-mortality, and aging is an irreversible risk factor of PAD. However, the effects of advanced age on IR-induced skeletal muscle mitochondrial dysfunction are not well known. Young and aged mice were therefore submitted to hindlimb IR (2 h ischemia followed by 2 h reperfusion). Skeletal muscle mitochondrial respiration, calcium retention capacity (CRC) and reactive oxygen species (ROS) production were determined using high resolution respirometry, spectrofluorometry and electronic paramagnetic resonance. IR-induced impairment in mitochondrial respiration was enhanced in old animals (V<sub>ADP</sub>; from 33.0 ± 2.4 to 18.4 ± 3.8 and 32.8 ± 1.3 to 5.9 ± 2.7 pmol/s/mg wet weight; −44.2 ± 11.4% vs. −82.0 ± 8.1%, in young and aged mice, respectively). Baseline CRC was lower in old animals and IR similarly decreased the CRC in both groups (from 11.8 ± 0.9 to 4.6 ± 0.9 and 5.5 ± 0.9 to 2.1 ± 0.3 µmol/mg dry weight; −60.9 ± 7.3 and −60.9 ± 4.6%, in young and aged mice, respectively). Further, IR-induced ROS production tended to be higher in aged mice. In conclusion, aging exacerbated the deleterious effects of IR on skeletal muscle mitochondrial respiration, potentially in relation to an increased oxidative stress.https://www.mdpi.com/2076-3921/8/6/168peripheral arterial diseaseischemia-reperfusionskeletal musclemitochondriarespirationcalciumoxidative stressreactive oxygen speciesaging |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Stéphanie Paradis Anne-Laure Charles Isabelle Georg Fabienne Goupilleau Alain Meyer Michel Kindo Gilles Laverny Daniel Metzger Bernard Geny |
spellingShingle |
Stéphanie Paradis Anne-Laure Charles Isabelle Georg Fabienne Goupilleau Alain Meyer Michel Kindo Gilles Laverny Daniel Metzger Bernard Geny Aging Exacerbates Ischemia-Reperfusion-Induced Mitochondrial Respiration Impairment in Skeletal Muscle Antioxidants peripheral arterial disease ischemia-reperfusion skeletal muscle mitochondria respiration calcium oxidative stress reactive oxygen species aging |
author_facet |
Stéphanie Paradis Anne-Laure Charles Isabelle Georg Fabienne Goupilleau Alain Meyer Michel Kindo Gilles Laverny Daniel Metzger Bernard Geny |
author_sort |
Stéphanie Paradis |
title |
Aging Exacerbates Ischemia-Reperfusion-Induced Mitochondrial Respiration Impairment in Skeletal Muscle |
title_short |
Aging Exacerbates Ischemia-Reperfusion-Induced Mitochondrial Respiration Impairment in Skeletal Muscle |
title_full |
Aging Exacerbates Ischemia-Reperfusion-Induced Mitochondrial Respiration Impairment in Skeletal Muscle |
title_fullStr |
Aging Exacerbates Ischemia-Reperfusion-Induced Mitochondrial Respiration Impairment in Skeletal Muscle |
title_full_unstemmed |
Aging Exacerbates Ischemia-Reperfusion-Induced Mitochondrial Respiration Impairment in Skeletal Muscle |
title_sort |
aging exacerbates ischemia-reperfusion-induced mitochondrial respiration impairment in skeletal muscle |
publisher |
MDPI AG |
series |
Antioxidants |
issn |
2076-3921 |
publishDate |
2019-06-01 |
description |
Cycles of ischemia-reperfusion (IR) that occur during peripheral arterial disease (PAD) are associated with significant morbi-mortality, and aging is an irreversible risk factor of PAD. However, the effects of advanced age on IR-induced skeletal muscle mitochondrial dysfunction are not well known. Young and aged mice were therefore submitted to hindlimb IR (2 h ischemia followed by 2 h reperfusion). Skeletal muscle mitochondrial respiration, calcium retention capacity (CRC) and reactive oxygen species (ROS) production were determined using high resolution respirometry, spectrofluorometry and electronic paramagnetic resonance. IR-induced impairment in mitochondrial respiration was enhanced in old animals (V<sub>ADP</sub>; from 33.0 ± 2.4 to 18.4 ± 3.8 and 32.8 ± 1.3 to 5.9 ± 2.7 pmol/s/mg wet weight; −44.2 ± 11.4% vs. −82.0 ± 8.1%, in young and aged mice, respectively). Baseline CRC was lower in old animals and IR similarly decreased the CRC in both groups (from 11.8 ± 0.9 to 4.6 ± 0.9 and 5.5 ± 0.9 to 2.1 ± 0.3 µmol/mg dry weight; −60.9 ± 7.3 and −60.9 ± 4.6%, in young and aged mice, respectively). Further, IR-induced ROS production tended to be higher in aged mice. In conclusion, aging exacerbated the deleterious effects of IR on skeletal muscle mitochondrial respiration, potentially in relation to an increased oxidative stress. |
topic |
peripheral arterial disease ischemia-reperfusion skeletal muscle mitochondria respiration calcium oxidative stress reactive oxygen species aging |
url |
https://www.mdpi.com/2076-3921/8/6/168 |
work_keys_str_mv |
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