Characterization of a recombinant thermotolerant argonaute protein as an endonuclease by broad guide utilization

Characterization of a recombinant thermotolerant argonaute protein as an endonuclease by broad guide utilization

Abstract Background Prokaryotic argonaute proteins (pAgos) play an important role in host defense in vivo. Most importantly, the thermophilic pAgos with endonuclease activity hold great potential for programmable genetic manipulation. Therefore, exploring argonaute proteins with unique enzyme proper...

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Bibliographic Details
Main Authors: Yuesheng Chong, Qian Liu, Fei Huang, Dong Song, Yan Feng
Format: Article
Language:English
Published: SpringerOpen 2019-06-01
Series:Bioresources and Bioprocessing
Subjects:
Argonaute protein
Nucleic acid guide
Endonuclease
DNA cleavage
Homologous modeling
Online Access:http://link.springer.com/article/10.1186/s40643-019-0254-8

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http://link.springer.com/article/10.1186/s40643-019-0254-8

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