CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes

CoronaHiT: high-throughput sequencing of SARS-CoV-2 genomes

Abstract We present CoronaHiT, a platform and throughput flexible method for sequencing SARS-CoV-2 genomes (≤ 96 on MinION or > 96 on Illumina NextSeq) depending on changing requirements experienced during the pandemic. CoronaHiT uses transposase-based library preparation of ARTIC PCR products. M...

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Bibliographic Details
Main Authors: Dave J. Baker, Alp Aydin, Thanh Le-Viet, Gemma L. Kay, Steven Rudder, Leonardo de Oliveira Martins, Ana P. Tedim, Anastasia Kolyva, Maria Diaz, Nabil-Fareed Alikhan, Lizzie Meadows, Andrew Bell, Ana Victoria Gutierrez, Alexander J. Trotter, Nicholas M. Thomson, Rachel Gilroy, Luke Griffith, Evelien M. Adriaenssens, Rachael Stanley, Ian G. Charles, Ngozi Elumogo, John Wain, Reenesh Prakash, Emma Meader, Alison E. Mather, Mark A. Webber, Samir Dervisevic, Andrew J. Page, Justin O’Grady
Format: Article
Language:English
Published: BMC 2021-02-01
Series:Genome Medicine
Subjects:
SARS-CoV-2
Nanopore
Sequencing
NGS
Genome
Genetic
Online Access:https://doi.org/10.1186/s13073-021-00839-5

Internet

https://doi.org/10.1186/s13073-021-00839-5

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