Development of a reference standard for the detection and quantification of Mycobacterium avium subsp. paratuberculosis by quantitative PCR

Development of a reference standard for the detection and quantification of Mycobacterium avium subsp. paratuberculosis by quantitative PCR

Abstract Quantitative PCR (qPCR) has become a frequently employed direct method for the detection and quantification of Mycobacterium avium subsp. paratuberculosis (MAP). The quantity of MAP determined by qPCR, however, may be affected by the type of qPCR quantification standard used (PCR product, p...

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Bibliographic Details
Main Authors: Monika Beinhauerova, Martina Beinhauerova, Sarah McCallum, Eric Sellal, Matteo Ricchi, Rory O’Brien, Beatrice Blanchard, Iva Slana, Vladimir Babak, Petr Kralik
Format: Article
Language:English
Published: Nature Publishing Group 2021-06-01
Series:Scientific Reports
Online Access:https://doi.org/10.1038/s41598-021-90789-0

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https://doi.org/10.1038/s41598-021-90789-0

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