Kinetic assay of starvation sensitivity in yeast autophagy mutants allows for the identification of intermediary phenotypes

Kinetic assay of starvation sensitivity in yeast autophagy mutants allows for the identification of intermediary phenotypes

Abstract Objective A classical method to quantitatively determine the starvation sensitivity phenotype of autophagy mutant budding yeast strains is to starve them for a period of time and then to assess the proportion of cells that retain the ability to form colonies when the availability of nutrien...

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Bibliographic Details
Main Authors: Candyce M. Sturgeon, Meaghan R. Robinson, Molly C. Penton, Deanna C. Clemmer, Maria A. Trujillo, Ambar U. Khawaja, Verónica A. Segarra
Format: Article
Language:English
Published: BMC 2019-08-01
Series:BMC Research Notes
Subjects:
Starvation sensitivity
96-well plate assay
High-throughput starvation sensitivity assay
Autophagy
Budding yeast
Atg27
Online Access:http://link.springer.com/article/10.1186/s13104-019-4545-0

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http://link.springer.com/article/10.1186/s13104-019-4545-0

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