A novel microfluidic flow-cytometry for counting numbers of single-cell β-actins

As a house keeping protein with stable expressions, β-actin is used as a loading control in normalization of western blotting. However, the actual numbers of β-actins at the single-cell level remain elusive. Based on a home-developed flow cytometry, single-cell numbers of β-actin from 8 cell types (...

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Bibliographic Details
Main Authors: Lixing Liu, Beiyuan Fan, Hongyu Yang, Deyong Chen, Shuang Zhang, Junbo Wang, Jian Chen
Format: Article
Language:English
Published: AIP Publishing LLC 2020-09-01
Series:Nanotechnology and Precision Engineering
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S2589554020300222