Shotgun metagenomes and multiple primer pair-barcode combinations of amplicons reveal biases in metabarcoding analyses of fungi
Rapid development of high-throughput (HTS) molecular identification methods has revolutionized our knowledge about taxonomic diversity and ecology of fungi. However, PCR-based methods exhibit multiple technical shortcomings that may bias our understanding of the fungal kingdom. This study was initia...
Main Authors: | Leho Tedersoo, Sten Anslan, Mohammad Bahram, Sergei Põlme, Taavi Riit, Ingrid Liiv, Urmas Kõljalg, Veljo Kisand, Henrik Nilsson, Falk Hildebrand, Peer Bork, Kessy Abarenkov |
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Format: | Article |
Language: | English |
Published: |
Pensoft Publishers
2015-05-01
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Series: | MycoKeys |
Online Access: | http://mycokeys.pensoft.net/lib/ajax_srv/article_elements_srv.php?action=download_pdf&item_id=4852 |
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