Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes In Vitro Assessment
The study was conducted to determine the antioxidant and anti-diabetic properties of bee pollen. The phenol content of extracts was 1.43, 2.04, 2.10 and 1.79 mg gallic acid g-1, respectively. Total flavonoid content was 0.78, 1.39, 0.86 and 0.79 mg gallic acid g-1 respectively. Bee pollen extracts h...
Main Author: | |
---|---|
Format: | Article |
Language: | English |
Published: |
Sciendo
2019-12-01
|
Series: | Journal of Apicultural Science |
Subjects: | |
Online Access: | https://doi.org/10.2478/jas-2019-0020 |
id |
doaj-995a56a555a7497d9cb5c928432b598e |
---|---|
record_format |
Article |
spelling |
doaj-995a56a555a7497d9cb5c928432b598e2021-09-06T19:41:31ZengSciendoJournal of Apicultural Science2299-48312019-12-0163231532510.2478/jas-2019-0020jas-2019-0020Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes In Vitro AssessmentDaudu Oluremi M.0Ahmadu Bello University, Zaria, NigeriaThe study was conducted to determine the antioxidant and anti-diabetic properties of bee pollen. The phenol content of extracts was 1.43, 2.04, 2.10 and 1.79 mg gallic acid g-1, respectively. Total flavonoid content was 0.78, 1.39, 0.86 and 0.79 mg gallic acid g-1 respectively. Bee pollen extracts have the potential to scavenge free radicals and lower blood glucose due to the presence of phenols and flavonoids. The aqueous-ethanol extract had the lowest IC50 for HRS and TAC, 0.53mg/mL and 0.25mg/mL respectively, which depicts that this extract alleviates the destructive effect of hydroxyl radicals. Methanol extract had the lowest IC50 0.21 mg/mL) for DPPH inhibition, hence it was able to scavenge the DPPH radicals. Ethanol extract had the lowest IC50 (0.04 mg/mL) for 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid inhibition, hence it can protect living cells from ABTS cations. The aqueous-ethanol extract displayed higher inhibition of α-amylase (4.51 mg/mL) while the aqueous extract exhibited a higher inhibition of α-glucosidase (0.60 mg/mL), which slows down the breakdown of disaccharides and reduces sugar buildup in the bloodstream. Bee pollen has potential antioxidative and antidiabetic activity.https://doi.org/10.2478/jas-2019-0020alpha-glucosidaseamylaseantidiabeticantioxidantbee-pollen |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Daudu Oluremi M. |
spellingShingle |
Daudu Oluremi M. Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes In Vitro Assessment Journal of Apicultural Science alpha-glucosidase amylase antidiabetic antioxidant bee-pollen |
author_facet |
Daudu Oluremi M. |
author_sort |
Daudu Oluremi M. |
title |
Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes In Vitro Assessment |
title_short |
Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes In Vitro Assessment |
title_full |
Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes In Vitro Assessment |
title_fullStr |
Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes In Vitro Assessment |
title_full_unstemmed |
Bee Pollen Extracts as Potential Antioxidants and Inhibitors of α-Amylase and α-Glucosidase Enzymes In Vitro Assessment |
title_sort |
bee pollen extracts as potential antioxidants and inhibitors of α-amylase and α-glucosidase enzymes in vitro assessment |
publisher |
Sciendo |
series |
Journal of Apicultural Science |
issn |
2299-4831 |
publishDate |
2019-12-01 |
description |
The study was conducted to determine the antioxidant and anti-diabetic properties of bee pollen. The phenol content of extracts was 1.43, 2.04, 2.10 and 1.79 mg gallic acid g-1, respectively. Total flavonoid content was 0.78, 1.39, 0.86 and 0.79 mg gallic acid g-1 respectively. Bee pollen extracts have the potential to scavenge free radicals and lower blood glucose due to the presence of phenols and flavonoids. The aqueous-ethanol extract had the lowest IC50 for HRS and TAC, 0.53mg/mL and 0.25mg/mL respectively, which depicts that this extract alleviates the destructive effect of hydroxyl radicals. Methanol extract had the lowest IC50 0.21 mg/mL) for DPPH inhibition, hence it was able to scavenge the DPPH radicals. Ethanol extract had the lowest IC50 (0.04 mg/mL) for 2, 2-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid inhibition, hence it can protect living cells from ABTS cations. The aqueous-ethanol extract displayed higher inhibition of α-amylase (4.51 mg/mL) while the aqueous extract exhibited a higher inhibition of α-glucosidase (0.60 mg/mL), which slows down the breakdown of disaccharides and reduces sugar buildup in the bloodstream. Bee pollen has potential antioxidative and antidiabetic activity. |
topic |
alpha-glucosidase amylase antidiabetic antioxidant bee-pollen |
url |
https://doi.org/10.2478/jas-2019-0020 |
work_keys_str_mv |
AT dauduoluremim beepollenextractsaspotentialantioxidantsandinhibitorsofaamylaseandaglucosidaseenzymesinvitroassessment |
_version_ |
1717766026021568512 |