Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens

Use of in-house PCR for identification of Mycobacterium tuberculosis in BACTEC broth cultures of respiratory specimens

We evaluated the ability of a PCR assay to identify Mycobacterium tuberculosis complex (MTBC) from positive BACTEC® 12B broth cultures. A total of 107 sputum samples were processed and inoculated into Ogawa slants and BACTEC® 12B vials. At a growth index (GI) > 30, 1.0 ml of the 12B broth was rem...

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Bibliographic Details
Main Authors: Ana Cláudia Carvalho Gouveia, Kathleen D. Eisenach, Solange Alves Vinhas, Fabiola Karla Corrêa Ribeiro, Renata Lyrio Peres, Reynaldo Dietze, David Jamil Hadad, Moisés Palaci
Format: Article
Language:English
Published: Instituto Oswaldo Cruz, Ministério da Saúde 2008-06-01
Series:Memórias do Instituto Oswaldo Cruz.
Subjects:
Mycobacterium tuberculosis
identification
PCR
BACTEC® 12B
culture
diagnosis
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000400012

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http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762008000400012

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