Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naïve human B cells
Abstract A successful HIV vaccine eliciting broadly neutralizing antibodies (bnAbs) must overcome the hurdle of being able to activate naive precursor B cells encoding features within their germline B cell receptors (BCR) that allow recognition of broadly neutralizing epitopes. Knowledge of whether...
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2021-09-01
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doaj-996c49bfefaf45c393289c1cfe6ce8532021-09-12T11:06:00ZengNature Publishing Groupnpj Vaccines2059-01052021-09-016111210.1038/s41541-021-00376-7Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naïve human B cellsJeong Hyun Lee0Laura Toy1Justin T. Kos2Yana Safonova3William R. Schief4Colin Havenar-Daughton5Corey T. Watson6Shane Crotty7Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI)Center for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI)Department of Biochemistry and Molecular Genetics, University of Louisville School of MedicineDepartment of Biochemistry and Molecular Genetics, University of Louisville School of MedicineConsortium for HIV/AIDS Vaccine Development (CHAVD), The Scripps Research InstituteCenter for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI)Department of Biochemistry and Molecular Genetics, University of Louisville School of MedicineCenter for Infectious Disease and Vaccine Research, La Jolla Institute for Immunology (LJI)Abstract A successful HIV vaccine eliciting broadly neutralizing antibodies (bnAbs) must overcome the hurdle of being able to activate naive precursor B cells encoding features within their germline B cell receptors (BCR) that allow recognition of broadly neutralizing epitopes. Knowledge of whether bnAb precursor B cells are circulating at sufficient frequencies within individuals in communities heavily impacted by HIV may be important. Using a germline-targeting eOD-GT8 immunogen and high-throughput droplet-based single-cell BCR sequencing, we demonstrate that large numbers of paired BCR sequences from multiple donors can be efficiently screened to elucidate precursor frequencies of rare, naive VRC01-class B cells. Further, we analyzed IGHV1-2 allelic usage among three different cohorts; we find that IGHV1-2 alleles traditionally thought to be incompatible with VRC01-class responses are relatively common in various human populations and that germline variation within IGHV1-2 associates with gene usage frequencies in the naive BCR repertoire.https://doi.org/10.1038/s41541-021-00376-7 |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Jeong Hyun Lee Laura Toy Justin T. Kos Yana Safonova William R. Schief Colin Havenar-Daughton Corey T. Watson Shane Crotty |
spellingShingle |
Jeong Hyun Lee Laura Toy Justin T. Kos Yana Safonova William R. Schief Colin Havenar-Daughton Corey T. Watson Shane Crotty Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naïve human B cells npj Vaccines |
author_facet |
Jeong Hyun Lee Laura Toy Justin T. Kos Yana Safonova William R. Schief Colin Havenar-Daughton Corey T. Watson Shane Crotty |
author_sort |
Jeong Hyun Lee |
title |
Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naïve human B cells |
title_short |
Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naïve human B cells |
title_full |
Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naïve human B cells |
title_fullStr |
Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naïve human B cells |
title_full_unstemmed |
Vaccine genetics of IGHV1-2 VRC01-class broadly neutralizing antibody precursor naïve human B cells |
title_sort |
vaccine genetics of ighv1-2 vrc01-class broadly neutralizing antibody precursor naïve human b cells |
publisher |
Nature Publishing Group |
series |
npj Vaccines |
issn |
2059-0105 |
publishDate |
2021-09-01 |
description |
Abstract A successful HIV vaccine eliciting broadly neutralizing antibodies (bnAbs) must overcome the hurdle of being able to activate naive precursor B cells encoding features within their germline B cell receptors (BCR) that allow recognition of broadly neutralizing epitopes. Knowledge of whether bnAb precursor B cells are circulating at sufficient frequencies within individuals in communities heavily impacted by HIV may be important. Using a germline-targeting eOD-GT8 immunogen and high-throughput droplet-based single-cell BCR sequencing, we demonstrate that large numbers of paired BCR sequences from multiple donors can be efficiently screened to elucidate precursor frequencies of rare, naive VRC01-class B cells. Further, we analyzed IGHV1-2 allelic usage among three different cohorts; we find that IGHV1-2 alleles traditionally thought to be incompatible with VRC01-class responses are relatively common in various human populations and that germline variation within IGHV1-2 associates with gene usage frequencies in the naive BCR repertoire. |
url |
https://doi.org/10.1038/s41541-021-00376-7 |
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