Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and Its Incorporation into Recombinant Proteins

Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and Its Incorporation into Recombinant Proteins

In this paper, we present a novel, “single experiment” methodology based on genetic engineering of metabolic pathways for direct intracellular production of non-canonical amino acids from simple precursors, coupled with expanded genetic code. In particular, we engineered the intracellular biosynth...

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Bibliographic Details
Main Authors: Ying Ma, Hernán Biava, Roberto Contestabile, Nediljko Budisa, Martino Luigi di Salvo
Format: Article
Language:English
Published: MDPI AG 2014-01-01
Series:Molecules
Subjects:
artificial metabolism/metabolic engineering
bioorthogonality
genetic code expansion
posttranslational modifications
L-methionine
L-azidohomoalanine
click chemistry
O-acetyl-L-homoserine sulfhydrylase
Online Access:http://www.mdpi.com/1420-3049/19/1/1004

Internet

http://www.mdpi.com/1420-3049/19/1/1004

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