Coupling Bioorthogonal Chemistries with Artificial Metabolism: Intracellular Biosynthesis of Azidohomoalanine and Its Incorporation into Recombinant Proteins
In this paper, we present a novel, “single experiment” methodology based on genetic engineering of metabolic pathways for direct intracellular production of non-canonical amino acids from simple precursors, coupled with expanded genetic code. In particular, we engineered the intracellular biosynth...
Main Authors: | Ying Ma, Hernán Biava, Roberto Contestabile, Nediljko Budisa, Martino Luigi di Salvo |
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Format: | Article |
Language: | English |
Published: |
MDPI AG
2014-01-01
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Series: | Molecules |
Subjects: | |
Online Access: | http://www.mdpi.com/1420-3049/19/1/1004 |
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