Enhanced Cas12a multi-gene regulation using a CRISPR array separator

Enhanced Cas12a multi-gene regulation using a CRISPR array separator

The type V-A Cas12a protein can process its CRISPR array, a feature useful for multiplexed gene editing and regulation. However, CRISPR arrays often exhibit unpredictable performance due to interference between multiple guide RNA (gRNAs). Here, we report that Cas12a array performance is hypersensiti...

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Bibliographic Details
Main Authors: Jens P Magnusson, Antonio Ray Rios, Lingling Wu, Lei S Qi
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2021-09-01
Series:eLife
Subjects:
CRISPR
Cas12a
multiplexed gene regulation
CRISPR array
separator
GC content
Online Access:https://elifesciences.org/articles/66406

Internet

https://elifesciences.org/articles/66406

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