Combining CRISPR-Cas12a-Based Technology and Metagenomics Next Generation Sequencing: A New Paradigm for Rapid and Full-Scale Detection of Microbes in Infectious Diabetic Foot Samples

Combining CRISPR-Cas12a-Based Technology and Metagenomics Next Generation Sequencing: A New Paradigm for Rapid and Full-Scale Detection of Microbes in Infectious Diabetic Foot Samples

Introduction: Diabetic foot infections (DFIs) pose a huge challenge for clinicians. Staphylococcus aureus, including methicillin-resistant S. aureus (MRSA), is one of the most significant pathogens of DFI. Early pathogen identification will greatly benefit the diagnosis and treatment of the disease....

Full description

Bibliographic Details
Main Authors: Yixin Chen, Ya Shi, Weifen Zhu, Jiaxing You, Jie Yang, Yaping Xie, Hanxin Zhao, Hongye Li, Shunwu Fan, Lin Li, Chao Liu
Format: Article
Language:English
Published: Frontiers Media S.A. 2021-10-01
Series:Frontiers in Microbiology
Subjects:
diabetic foot infections
Staphylococcus aureus
loop-mediated isothermal amplification
clustered regularly interspaced short palindromic repeats
metagenomics next generation sequencing
Online Access:https://www.frontiersin.org/articles/10.3389/fmicb.2021.742040/full

Internet

https://www.frontiersin.org/articles/10.3389/fmicb.2021.742040/full

Similar Items