A simple, rapid, and sensitive fluorescence-based method to assess triacylglycerol hydrolase activity

Lipases constitute an important class of water-soluble enzymes that catalyze the hydrolysis of hydrophobic triacylglycerol (TAG). Their enzymatic activity is typically measured using multistep procedures involving isolation and quantification of the hydrolyzed products. We report here a new fluoresc...

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Bibliographic Details
Main Authors: Sujith Rajan, Hazel C. de Guzman, Thomas Palaia, Ira J. Goldberg, M. Mahmood Hussain
Format: Article
Language:English
Published: Elsevier 2021-01-01
Series:Journal of Lipid Research
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S0022227521000973