Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells
Regulatory T (Treg) cells mainly develop within the thymus and arise from CD25+Foxp3− (CD25+ TregP) or CD25−Foxp3+ (Foxp3+ TregP) Treg cell precursors resulting in Treg cells harboring distinct transcriptomic profiles and complementary T cell receptor repertoires. The stable and long-term expression...
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doaj-9a5401e312c7446ebd3e9fe0ba2aedd42020-11-25T02:12:54ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-10-011010.3389/fimmu.2019.02382487962Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T CellsSusanne Herppich0Aras Toker1Beate Pietzsch2Yohko Kitagawa3Naganari Ohkura4Takahisa Miyao5Stefan Floess6Shohei Hori7Shohei Hori8Shimon Sakaguchi9Jochen Huehn10Department Experimental Immunology, Helmholtz Centre for Infection Research, Braunschweig, GermanyDepartment Experimental Immunology, Helmholtz Centre for Infection Research, Braunschweig, GermanyDepartment Experimental Immunology, Helmholtz Centre for Infection Research, Braunschweig, GermanyLaboratory of Experimental Immunology, World Premier International Research Center Immunology Frontier Research Center, Osaka University, Osaka, JapanLaboratory of Experimental Immunology, World Premier International Research Center Immunology Frontier Research Center, Osaka University, Osaka, JapanLaboratory for Immune Homeostasis, RIKEN Center for Integrative Medical Sciences, Yokohama, JapanDepartment Experimental Immunology, Helmholtz Centre for Infection Research, Braunschweig, GermanyRIKEN Center for Integrative Medical Sciences, Yokohama, JapanLaboratory of Immunology and Microbiology, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Tokyo, JapanLaboratory of Experimental Immunology, World Premier International Research Center Immunology Frontier Research Center, Osaka University, Osaka, JapanDepartment Experimental Immunology, Helmholtz Centre for Infection Research, Braunschweig, GermanyRegulatory T (Treg) cells mainly develop within the thymus and arise from CD25+Foxp3− (CD25+ TregP) or CD25−Foxp3+ (Foxp3+ TregP) Treg cell precursors resulting in Treg cells harboring distinct transcriptomic profiles and complementary T cell receptor repertoires. The stable and long-term expression of Foxp3 in Treg cells and their stable suppressive phenotype are controlled by the demethylation of Treg cell-specific epigenetic signature genes including an evolutionarily conserved CpG-rich element within the Foxp3 locus, the Treg-specific demethylated region (TSDR). Here we analyzed the dynamics of the imprinting of the Treg cell-specific epigenetic signature genes in thymic Treg cells. We could demonstrate that CD25+Foxp3+ Treg cells show a progressive demethylation of most signature genes during maturation within the thymus. Interestingly, a partial demethylation of several Treg cell-specific epigenetic signature genes was already observed in Foxp3+ TregP but not in CD25+ TregP. Furthermore, Foxp3+ TregP were very transient in nature and arose at a more mature developmental stage when compared to CD25+ TregP. When the two Treg cell precursors were cultured in presence of IL-2, a factor known to be critical for thymic Treg cell development, we observed a major impact of IL-2 on the demethylation of the TSDR with a more pronounced effect on Foxp3+ TregP. Together, these results suggest that the establishment of the Treg cell-specific hypomethylation pattern is a continuous process throughout thymic Treg cell development and that the two known Treg cell precursors display distinct dynamics for the imprinting of the Treg cell-specific epigenetic signature genes.https://www.frontiersin.org/article/10.3389/fimmu.2019.02382/fullTreg cellTreg cell precursorsdemethylationepigenetic signatureIL-2thymus |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
Susanne Herppich Aras Toker Beate Pietzsch Yohko Kitagawa Naganari Ohkura Takahisa Miyao Stefan Floess Shohei Hori Shohei Hori Shimon Sakaguchi Jochen Huehn |
spellingShingle |
Susanne Herppich Aras Toker Beate Pietzsch Yohko Kitagawa Naganari Ohkura Takahisa Miyao Stefan Floess Shohei Hori Shohei Hori Shimon Sakaguchi Jochen Huehn Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells Frontiers in Immunology Treg cell Treg cell precursors demethylation epigenetic signature IL-2 thymus |
author_facet |
Susanne Herppich Aras Toker Beate Pietzsch Yohko Kitagawa Naganari Ohkura Takahisa Miyao Stefan Floess Shohei Hori Shohei Hori Shimon Sakaguchi Jochen Huehn |
author_sort |
Susanne Herppich |
title |
Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells |
title_short |
Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells |
title_full |
Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells |
title_fullStr |
Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells |
title_full_unstemmed |
Dynamic Imprinting of the Treg Cell-Specific Epigenetic Signature in Developing Thymic Regulatory T Cells |
title_sort |
dynamic imprinting of the treg cell-specific epigenetic signature in developing thymic regulatory t cells |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Immunology |
issn |
1664-3224 |
publishDate |
2019-10-01 |
description |
Regulatory T (Treg) cells mainly develop within the thymus and arise from CD25+Foxp3− (CD25+ TregP) or CD25−Foxp3+ (Foxp3+ TregP) Treg cell precursors resulting in Treg cells harboring distinct transcriptomic profiles and complementary T cell receptor repertoires. The stable and long-term expression of Foxp3 in Treg cells and their stable suppressive phenotype are controlled by the demethylation of Treg cell-specific epigenetic signature genes including an evolutionarily conserved CpG-rich element within the Foxp3 locus, the Treg-specific demethylated region (TSDR). Here we analyzed the dynamics of the imprinting of the Treg cell-specific epigenetic signature genes in thymic Treg cells. We could demonstrate that CD25+Foxp3+ Treg cells show a progressive demethylation of most signature genes during maturation within the thymus. Interestingly, a partial demethylation of several Treg cell-specific epigenetic signature genes was already observed in Foxp3+ TregP but not in CD25+ TregP. Furthermore, Foxp3+ TregP were very transient in nature and arose at a more mature developmental stage when compared to CD25+ TregP. When the two Treg cell precursors were cultured in presence of IL-2, a factor known to be critical for thymic Treg cell development, we observed a major impact of IL-2 on the demethylation of the TSDR with a more pronounced effect on Foxp3+ TregP. Together, these results suggest that the establishment of the Treg cell-specific hypomethylation pattern is a continuous process throughout thymic Treg cell development and that the two known Treg cell precursors display distinct dynamics for the imprinting of the Treg cell-specific epigenetic signature genes. |
topic |
Treg cell Treg cell precursors demethylation epigenetic signature IL-2 thymus |
url |
https://www.frontiersin.org/article/10.3389/fimmu.2019.02382/full |
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