T-cell calcium dynamics visualized in a ratiometric tdTomato-GCaMP6f transgenic reporter mouse

Calcium is an essential cellular messenger that regulates numerous functions in living organisms. Here, we describe development and characterization of ‘Salsa6f’, a fusion of GCaMP6f and tdTomato optimized for cell tracking while monitoring cytosolic Ca2+, and a transgenic Ca2+ reporter mouse with S...

Full description

Bibliographic Details
Main Authors: Tobias X Dong, Shivashankar Othy, Amit Jairaman, Jonathan Skupsky, Angel Zavala, Ian Parker, Joseph L Dynes, Michael D Cahalan
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2017-12-01
Series:eLife
Subjects:
Online Access:https://elifesciences.org/articles/32417