| Summary: | The present work deals with the delignification of wheat straw and sugarcane bagasse with different pretreatmenttechniques followed by saccharification with commercial and indigenously produced cellulase enzymes. 100 g of sugar canebagasse and whaet straw were treated with various concentrations of H2O2 (1-5% v/v) and its combination with 2% NaOHfor one hour under steam at 30 psi. Sugarcane bagasse was 88% delignified with 5% H2O2 + 2% NaOH whereas wheat strawshowed 51% delignification with 3% H2O2 + 2% NaOH. Moreover the saccharification with commercial cellulase enzyme wasfound to be 33.6% and 63.3% with pretreated wheat straw and sugarcane bagasse, respectively. However, very low levels ofsaccharification (6-14%) were found with indigenously produced cellulase enzyme.
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