Quantitative assay for TALEN activity at endogenous genomic loci

Quantitative assay for TALEN activity at endogenous genomic loci

Summary Artificially designed nucleases such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) can induce a targeted DNA double-strand break at the specific target genomic locus, leading to the frameshift-mediated gene disruption. However, the assays for th...

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Bibliographic Details
Main Authors: Yu Hisano, Satoshi Ota, Kazuharu Arakawa, Michiko Muraki, Nobuaki Kono, Kazuki Oshita, Tetsushi Sakuma, Masaru Tomita, Takashi Yamamoto, Yasushi Okada, Atsuo Kawahara
Format: Article
Language:English
Published: The Company of Biologists 2013-02-01
Series:Biology Open
Subjects:
TALEN
LacZ blue–white selection
Quantitative assay
Genome editing
Online Access:http://bio.biologists.org/content/2/4/363

Internet

http://bio.biologists.org/content/2/4/363

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