Quantitative assay for TALEN activity at endogenous genomic loci
Summary Artificially designed nucleases such as zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs) can induce a targeted DNA double-strand break at the specific target genomic locus, leading to the frameshift-mediated gene disruption. However, the assays for th...
Main Authors: | , , , , , , , , , , |
---|---|
Format: | Article |
Language: | English |
Published: |
The Company of Biologists
2013-02-01
|
Series: | Biology Open |
Subjects: | |
Online Access: | http://bio.biologists.org/content/2/4/363 |