Manipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency

Manipulating the position of DNA expression cassettes using location tags fused to dCas9 (Cas9-Lag) to improve metabolic pathway efficiency

Abstract Background Deactivated Cas9 (dCas9) led to significant improvement of CRISPR/Cas9-based techniques because it can be fused with a variety of functional groups to form diverse molecular devices, which can manipulate or modify target DNA cassettes. One important metabolic engineering strategy...

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Bibliographic Details
Main Authors: Qianwen Xie, Siwei Li, Dongdong Zhao, Lijun Ye, Qingyan Li, Xueli Zhang, Li Zhu, Changhao Bi
Format: Article
Language:English
Published: BMC 2020-12-01
Series:Microbial Cell Factories
Subjects:
dCas9
Complex localization
Astaxanthin
Carotenoids
Escherichia coli
Online Access:https://doi.org/10.1186/s12934-020-01496-w

Internet

https://doi.org/10.1186/s12934-020-01496-w

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