Conversion of an inactive xylose isomerase into a functional enzyme by co-expression of GroEL-GroES chaperonins in Saccharomyces cerevisiae

Conversion of an inactive xylose isomerase into a functional enzyme by co-expression of GroEL-GroES chaperonins in Saccharomyces cerevisiae

Abstract Background Second-generation ethanol production is a clean bioenergy source with potential to mitigate fossil fuel emissions. The engineering of Saccharomyces cerevisiae for xylose utilization is an essential step towards the production of this biofuel. Though xylose isomerase (XI) is the k...

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Bibliographic Details
Main Authors: Beatriz Temer, Leandro Vieira dos Santos, Victor Augusti Negri, Juliana Pimentel Galhardo, Pedro Henrique Mello Magalhães, Juliana José, Cidnei Marschalk, Thamy Lívia Ribeiro Corrêa, Marcelo Falsarella Carazzolle, Gonçalo Amarante Guimarães Pereira
Format: Article
Language:English
Published: BMC 2017-09-01
Series:BMC Biotechnology
Subjects:
Xylose isomerase
Saccharomyces cerevisiae
Xylose fermentation
GroEL-GroES chaperonins
Ethanol production
Online Access:http://link.springer.com/article/10.1186/s12896-017-0389-7

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http://link.springer.com/article/10.1186/s12896-017-0389-7

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