An MHV-68 Mutator Phenotype Mutant Virus, Confirmed by CRISPR/Cas9-Mediated Gene Editing of the Viral DNA Polymerase Gene, Shows Reduced Viral Fitness

An MHV-68 Mutator Phenotype Mutant Virus, Confirmed by CRISPR/Cas9-Mediated Gene Editing of the Viral DNA Polymerase Gene, Shows Reduced Viral Fitness

Drug resistance studies on human γ-herpesviruses are hampered by the absence of an in vitro system that allows efficient lytic viral replication. Therefore, we employed murine γ-herpesvirus-68 (MHV-68) that efficiently replicates in vitro as a model to study the antiviral resistance of γ-herpesvirus...

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Bibliographic Details
Main Authors: Erika Trompet, Arturo Temblador, Sarah Gillemot, Dimitrios Topalis, Robert Snoeck, Graciela Andrei
Format: Article
Language:English
Published: MDPI AG 2021-05-01
Series:Viruses
Subjects:
herpesviruses 1
DNA polymerase 2
drug resistance 3
mutator phenotype 4
viral fitness 5
CRISPR/Cas9 precise gene editing
Online Access:https://www.mdpi.com/1999-4915/13/6/985

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https://www.mdpi.com/1999-4915/13/6/985

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