Culture of human pluripotent stem cells using completely defined conditions on a recombinant E-cadherin substratum

<p>Abstract</p> <p>Background</p> <p>To maintain pluripotency of human embryonic stem (huES) cells in feeder-free culture it has been necessary to provide a Matrigel substratum, which is a complex of poorly defined extracellular matrices and growth factors derived from...

Full description

Bibliographic Details
Main Authors: Duncan Stephen A, Akaike Toshihiro, Si-Tayeb Karim, Nagaoka Masato
Format: Article
Language:English
Published: BMC 2010-06-01
Series:BMC Developmental Biology
Online Access:http://www.biomedcentral.com/1471-213X/10/60