Localization of MTT formazan in lipid droplets. An alternative hypothesis about the nature of formazan granules and aggregates

MTT (3-(4, 5-dimethyl-2-thiazolyl)-2, 5-dihphenyltetrazolium bromide) assay is a widely used method to assess cell viability and proliferation. MTT is readily taken up by cells and enzymatically reduced to formazan, a dark compound which accumulates in cytoplasmic granules. Formazan is later elimina...

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Bibliographic Details
Main Authors: G Diaz, M Melis, A Musinu, M Piludu, M Piras, AM Falchi
Format: Article
Language:English
Published: PAGEPress Publications 2009-08-01
Series:European Journal of Histochemistry
Online Access:http://www.ejh.it/index.php/ejh/article/view/1145