Evolution of BRET biosensors from live cell to tissue-scale in vivo imaging
Development of bioluminescence resonance energy transfer [BRET] based genetic sensors for sensing biological functions such as protein-protein interactions [PPIs] in vivo has a special value in measuring such dynamic events at their native environment. Since its inception in the late nineties, BRET...
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2013-09-01
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doaj-9c6334d7c7fe43489a370d446b4c48bc2020-11-24T23:05:06ZengFrontiers Media S.A.Frontiers in Endocrinology1664-23922013-09-01410.3389/fendo.2013.0013160009Evolution of BRET biosensors from live cell to tissue-scale in vivo imagingABHIJIT eDE0Akshi eJasani1Rohit eArora2Sanjiv Sam Gambhir3ACTREC, Tata Memorial CentreACTREC, Tata Memorial CentreACTREC, Tata Memorial CentreStanford UniversityDevelopment of bioluminescence resonance energy transfer [BRET] based genetic sensors for sensing biological functions such as protein-protein interactions [PPIs] in vivo has a special value in measuring such dynamic events at their native environment. Since its inception in the late nineties, BRET related research has gained significant momentum in terms of adding versatility to the assay format and wider applicability where it has been suitably used. Beyond the scope of quantitative measurement of PPIs and protein dimerization, molecular imaging applications based on BRET assays have broadened its scope for screening pharmacologically important compounds by in vivo imaging as well. In this mini-review we focus on an in-depth analysis of engineered BRET systems developed and their successful application to cell-based assays as well as in vivo noninvasive imaging in live subjects.http://journal.frontiersin.org/Journal/10.3389/fendo.2013.00131/fulloptical imagingbioluminescence resonance energy transferprotein-protein interactionsfluorescent proteinsluciferaseCell-based assay |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
ABHIJIT eDE Akshi eJasani Rohit eArora Sanjiv Sam Gambhir |
spellingShingle |
ABHIJIT eDE Akshi eJasani Rohit eArora Sanjiv Sam Gambhir Evolution of BRET biosensors from live cell to tissue-scale in vivo imaging Frontiers in Endocrinology optical imaging bioluminescence resonance energy transfer protein-protein interactions fluorescent proteins luciferase Cell-based assay |
author_facet |
ABHIJIT eDE Akshi eJasani Rohit eArora Sanjiv Sam Gambhir |
author_sort |
ABHIJIT eDE |
title |
Evolution of BRET biosensors from live cell to tissue-scale in vivo imaging |
title_short |
Evolution of BRET biosensors from live cell to tissue-scale in vivo imaging |
title_full |
Evolution of BRET biosensors from live cell to tissue-scale in vivo imaging |
title_fullStr |
Evolution of BRET biosensors from live cell to tissue-scale in vivo imaging |
title_full_unstemmed |
Evolution of BRET biosensors from live cell to tissue-scale in vivo imaging |
title_sort |
evolution of bret biosensors from live cell to tissue-scale in vivo imaging |
publisher |
Frontiers Media S.A. |
series |
Frontiers in Endocrinology |
issn |
1664-2392 |
publishDate |
2013-09-01 |
description |
Development of bioluminescence resonance energy transfer [BRET] based genetic sensors for sensing biological functions such as protein-protein interactions [PPIs] in vivo has a special value in measuring such dynamic events at their native environment. Since its inception in the late nineties, BRET related research has gained significant momentum in terms of adding versatility to the assay format and wider applicability where it has been suitably used. Beyond the scope of quantitative measurement of PPIs and protein dimerization, molecular imaging applications based on BRET assays have broadened its scope for screening pharmacologically important compounds by in vivo imaging as well. In this mini-review we focus on an in-depth analysis of engineered BRET systems developed and their successful application to cell-based assays as well as in vivo noninvasive imaging in live subjects. |
topic |
optical imaging bioluminescence resonance energy transfer protein-protein interactions fluorescent proteins luciferase Cell-based assay |
url |
http://journal.frontiersin.org/Journal/10.3389/fendo.2013.00131/full |
work_keys_str_mv |
AT abhijitede evolutionofbretbiosensorsfromlivecelltotissuescaleinvivoimaging AT akshiejasani evolutionofbretbiosensorsfromlivecelltotissuescaleinvivoimaging AT rohitearora evolutionofbretbiosensorsfromlivecelltotissuescaleinvivoimaging AT sanjivsamgambhir evolutionofbretbiosensorsfromlivecelltotissuescaleinvivoimaging |
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