Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases

Using mutability landscapes of a promiscuous tautomerase to guide the engineering of enantioselective Michaelases

The Michael-type addition reaction is used for carbon-carbon bond formation; however biocatalytic methods for this reaction are rare. Here, the authors generate and exploit mutability landscapes of 4-oxalocrotonate tautomerase to direct the redesign of this promiscuous enzyme into enantio-complement...

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Bibliographic Details
Main Authors: Jan-Ytzen van der Meer, Harshwardhan Poddar, Bert-Jan Baas, Yufeng Miao, Mehran Rahimi, Andreas Kunzendorf, Ronald van Merkerk, Pieter G. Tepper, Edzard M. Geertsema, Andy-Mark W. H. Thunnissen, Wim J. Quax, Gerrit J. Poelarends
Format: Article
Language:English
Published: Nature Publishing Group 2016-03-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/ncomms10911

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https://doi.org/10.1038/ncomms10911

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