Longitudinal imaging of HIV-1 spread in humanized mice with parallel 3D immunofluorescence and electron tomography

Longitudinal imaging of HIV-1 spread in humanized mice with parallel 3D immunofluorescence and electron tomography

Dissemination of HIV-1 throughout lymphoid tissues leads to systemic virus spread following infection. We combined tissue clearing, 3D-immunofluorescence, and electron tomography (ET) to longitudinally assess early HIV-1 spread in lymphoid tissues in humanized mice. Immunofluorescence revealed peak...

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Bibliographic Details
Main Authors: Collin Kieffer, Mark S Ladinsky, Allen Ninh, Rachel P Galimidi, Pamela J Bjorkman
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2017-02-01
Series:eLife
Subjects:
humanized mouse
tissue clearing
HIV-1
electron tomography
3D immunofluorescence
CLARITY
Online Access:https://elifesciences.org/articles/23282

Internet

https://elifesciences.org/articles/23282

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