Ubiquitination-induced fluorescence complementation (UiFC) for detection of K48 ubiquitin chains in vitro and in live cells.

Ubiquitination-induced fluorescence complementation (UiFC) for detection of K48 ubiquitin chains in vitro and in live cells.

Proteins can be modified with eight homogenous ubiquitin chains linked by an isopeptide bond between the C-terminus of one ubiquitin and an amine from one of the seven lysines or the N-terminal methionine of the next ubiquitin. These topologically distinct ubiquitin chains signal for many essential...

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Bibliographic Details
Main Authors: Zhiliang Chen, Yongwang Zhong, Yang Wang, Shan Xu, Zheng Liu, Ilia V Baskakov, Mervyn J Monteiro, Mariusz Karbowski, Yuxian Shen, Shengyun Fang
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2013-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3764048?pdf=render

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http://europepmc.org/articles/PMC3764048?pdf=render

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