An Optimized Chromatin Immunoprecipitation Protocol for Quantification of Protein-DNA Interactions

Summary: Transcription factors are important regulators of cell fate and function. Knowledge about where transcription factors are bound in the genome is crucial for understanding their function. A common method to study protein-DNA interactions is chromatin immunoprecipitation (ChIP). Here, we pres...

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Bibliographic Details
Main Authors: Wim J. de Jonge, Mariël Brok, Patrick Kemmeren, Frank C.P. Holstege
Format: Article
Language:English
Published: Elsevier 2020-06-01
Series:STAR Protocols
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166720300071