cAMP-PKA dependent ERK1/2 activation is necessary for vanillic acid potentiated glucose-stimulated insulin secretion in pancreatic β-cells
Vanillic acid (VA), a dietary phenolic compound is generally studied for its anti-oxidative and anti-inflammatory effects. However, the effect of VA on insulin secretion and its mechanism of action has never been explored. In this study, we report that VA augments glucose-stimulated insulin secretio...
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doaj-9d9c0c4de1024aa9b82ea6b39785b5582021-04-30T07:15:57ZengElsevierJournal of Functional Foods1756-46462019-05-0156110118cAMP-PKA dependent ERK1/2 activation is necessary for vanillic acid potentiated glucose-stimulated insulin secretion in pancreatic β-cellsV.P. Mahendra0Devendra J. Haware1Ravi Kumar2Department of Molecular Nutrition, CSIR-Central Food Technological Research Institute (CFTRI), Mysore 570 020, Karnataka, India; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad 201002, IndiaDepartment of Food Safety and Analytical Quality Control Laboratory, CSIR-Central Food Technological Research Institute (CFTRI), Mysore 570 020, Karnataka, IndiaDepartment of Molecular Nutrition, CSIR-Central Food Technological Research Institute (CFTRI), Mysore 570 020, Karnataka, India; Academy of Scientific & Innovative Research (AcSIR), Ghaziabad 201002, India; Corresponding author at: Department of Molecular Nutrition, CSIR-Central Food Technological Research Institute (CFTRI), Mysore 570 020, Karnataka, India.Vanillic acid (VA), a dietary phenolic compound is generally studied for its anti-oxidative and anti-inflammatory effects. However, the effect of VA on insulin secretion and its mechanism of action has never been explored. In this study, we report that VA augments glucose-stimulated insulin secretion (GSIS) in both insulin-secreting cell-line INS-1 and isolated rat pancreatic islets. Potentiation of GSIS is accompanied by a concurrent increase in 3′,5′-cyclic adenosine monophosphate (cAMP) and activation of protein kinase A (PKA) in INS-1 and rat islets. The activated cAMP-PKA pathway, in turn, phosphorylates extracellular signal-regulated kinases 1/2 (ERK1/2) in INS-1 cells. Pharmacological intervention with PKA and ERK1/2 inhibitors revealed that VA potentiated GSIS is primarily dependent on PKA mediated ERK1/2 activity. These findings demonstrated that VA directly acts on insulin-secreting pancreatic β-cells to exert its insulinotropic effect thereby providing a novel role of VA in the regulation of insulin secretion.http://www.sciencedirect.com/science/article/pii/S1756464619301197Vanillic acidPKAGSISERK1/2Pancreatic β-cellscAMP |
collection |
DOAJ |
language |
English |
format |
Article |
sources |
DOAJ |
author |
V.P. Mahendra Devendra J. Haware Ravi Kumar |
spellingShingle |
V.P. Mahendra Devendra J. Haware Ravi Kumar cAMP-PKA dependent ERK1/2 activation is necessary for vanillic acid potentiated glucose-stimulated insulin secretion in pancreatic β-cells Journal of Functional Foods Vanillic acid PKA GSIS ERK1/2 Pancreatic β-cells cAMP |
author_facet |
V.P. Mahendra Devendra J. Haware Ravi Kumar |
author_sort |
V.P. Mahendra |
title |
cAMP-PKA dependent ERK1/2 activation is necessary for vanillic acid potentiated glucose-stimulated insulin secretion in pancreatic β-cells |
title_short |
cAMP-PKA dependent ERK1/2 activation is necessary for vanillic acid potentiated glucose-stimulated insulin secretion in pancreatic β-cells |
title_full |
cAMP-PKA dependent ERK1/2 activation is necessary for vanillic acid potentiated glucose-stimulated insulin secretion in pancreatic β-cells |
title_fullStr |
cAMP-PKA dependent ERK1/2 activation is necessary for vanillic acid potentiated glucose-stimulated insulin secretion in pancreatic β-cells |
title_full_unstemmed |
cAMP-PKA dependent ERK1/2 activation is necessary for vanillic acid potentiated glucose-stimulated insulin secretion in pancreatic β-cells |
title_sort |
camp-pka dependent erk1/2 activation is necessary for vanillic acid potentiated glucose-stimulated insulin secretion in pancreatic β-cells |
publisher |
Elsevier |
series |
Journal of Functional Foods |
issn |
1756-4646 |
publishDate |
2019-05-01 |
description |
Vanillic acid (VA), a dietary phenolic compound is generally studied for its anti-oxidative and anti-inflammatory effects. However, the effect of VA on insulin secretion and its mechanism of action has never been explored. In this study, we report that VA augments glucose-stimulated insulin secretion (GSIS) in both insulin-secreting cell-line INS-1 and isolated rat pancreatic islets. Potentiation of GSIS is accompanied by a concurrent increase in 3′,5′-cyclic adenosine monophosphate (cAMP) and activation of protein kinase A (PKA) in INS-1 and rat islets. The activated cAMP-PKA pathway, in turn, phosphorylates extracellular signal-regulated kinases 1/2 (ERK1/2) in INS-1 cells. Pharmacological intervention with PKA and ERK1/2 inhibitors revealed that VA potentiated GSIS is primarily dependent on PKA mediated ERK1/2 activity. These findings demonstrated that VA directly acts on insulin-secreting pancreatic β-cells to exert its insulinotropic effect thereby providing a novel role of VA in the regulation of insulin secretion. |
topic |
Vanillic acid PKA GSIS ERK1/2 Pancreatic β-cells cAMP |
url |
http://www.sciencedirect.com/science/article/pii/S1756464619301197 |
work_keys_str_mv |
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1721499112111603712 |